RT Journal Article
SR Electronic
T1 Renieramycin M Attenuates Cancer Stem Cell-like Phenotypes in H460 Lung Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 615
OP 621
VO 37
IS 2
A1 SIRIMANGKALAKITTI, NATCHANUN
A1 CHAMNI, SUPAKARN
A1 SUWANBORIRUX, KHANIT
A1 CHANVORACHOTE, PITHI
YR 2017
UL http://ar.iiarjournals.org/content/37/2/615.abstract
AB Background/Aim: Cancer stem cells (CSCs) are a subpopulation of cancer cells that possess self-renewal and differentiation capacities. CSCs contribute to drug-resistance, cancer recurrence and metastasis, thus development of CSC-targeted therapeutic strategies has recently received significant attention in cancer research. In this study, the potential efficacy of renieramycin M (RM) isolated from the sponge Xestospongia species, was examined against lung CSCs. Materials and Methods: Colony and spheroid formation assays, as well as western blotting analysis of lung CSC protein markers were employed to determine the CSC-like phenotypes of H460 lung cancer cells after treatment with RM at non-toxic concentrations. Results: RM treatment reduced significantly colony and spheroid formation of H460 cells. Moreover, the CSC markers CD133, CD44 and ALDH1A1 of CSC-enriched H460 cells were reduced significantly following RM treatment. Conclusion: RM could be a potent anti-metastatic agent by suppressing lung CSC-like phenotypes in H460 cells.