RT Journal Article
SR Electronic
T1 Inhibition of Glutaminolysis Inhibits Cell Growth <em>via</em> Down-regulating Mtorc1 Signaling in Lung Squamous Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6021
OP 6029
VO 36
IS 11
A1 XULU YE
A1 QILIANG ZHOU
A1 YOSHIFUMI MATSUMOTO
A1 MASATO MORIYAMA
A1 SHUN KAGEYAMA
A1 MASAAKI KOMATSU
A1 SEIJIRO SATOH
A1 MASANORI TSUCHIDA
A1 YASUO SAIJO
YR 2016
UL http://ar.iiarjournals.org/content/36/11/6021.abstract
AB Background/Aim: Inhibition of glutaminolysis has been reported as a promising therapeutic strategy to target several solid carcinomas. We aimed to investigate the effects of glutaminolysis on cell proliferation in lung squamous cell carcinoma cell lines and to explore the potential of targeting glutaminolysis as an anticancer strategy. Materials and Methods: Glutamine (Gln) dependence was assessed in six lung squamous cell carcinoma cell lines. Cell proliferation, mammalian target of rapamycin complex 1 (mTORC1) activity and the induction of autophagy were assessed after inhibition of glutaminolysis via Gln depletion or glutaminase (GLS) inhibition. Results: Five of six lung squamous cell carcinoma cell lines exhibited glutamine-dependence. The extent of dependence was correlated with the mRNA levels of GLS1/GLS2. Inhibition of glutaminolysis inhibited cell proliferation by down-regulating of mTORC1 signaling and inducing autophagy in Gln-dependent lung squamous cell carcinoma cell lines. Conclusion: Targeting glutaminolysis may represent a potential therapeutic strategy for the treatment of Gln-dependent lung squamous cell carcinomas.