PT - JOURNAL ARTICLE AU - YU KAMATA AU - TOMOKI SUMIDA AU - RYUICHI MURASE AU - HIROYUKI NAKANO AU - TOMOHIRO YAMADA AU - YOSHIHIDE MORI TI - Blockade of Androgen-induced Malignant Phenotypes by Flutamide Administration in Human Salivary Duct Carcinoma Cells DP - 2016 Nov 01 TA - Anticancer Research PG - 6071--6075 VI - 36 IP - 11 4099 - http://ar.iiarjournals.org/content/36/11/6071.short 4100 - http://ar.iiarjournals.org/content/36/11/6071.full SO - Anticancer Res2016 Nov 01; 36 AB - Background/Aim: Androgens are known to play a critical role in prostate cancer progression, but their effect on malignant phenotypes in salivary gland cancer is unclear. The androgen–androgen receptor (AR) axis may be involved in malignant phenotypes of salivary duct carcinoma (SDC) cells and therefore may be a new target for SDC treatment. To test this hypothesis, we investigated the effect of the androgen 5α-dihydrotestosterone (DHT) on proliferation, migration, and invasiveness of SDC cells. Materials and Methods: We used a wound-healing assay to measure cell migration and a Boyden chamber invasion assay to investigate SDC cell invasive capacity. Results: DHT treatment increased cell proliferation, migration, and invasion. However, treatment with flutamide, an AR inhibitor, blocked the effects of DHT. Conclusion: These results suggest that the androgen–AR axis is involved in SDC malignancy and may be an effective therapeutic target for treatment of human SDC.