PT  - JOURNAL ARTICLE
AU  - MARIA TOLOUDI
AU  - MARIA PAPADIMITRIOU
AU  - KATERINA PANTOPIKOU
AU  - IOANNIS PAPASOTIRIOU
TI  - Generation of Human Antibodies Against a Specific Peptide: a Novel Strategy Based on Human Cells
DP  - 2016 Sep 01
TA  - Anticancer Research
PG  - 4613--4621
VI  - 36
IP  - 9
4099  - http://ar.iiarjournals.org/content/36/9/4613.short
4100  - http://ar.iiarjournals.org/content/36/9/4613.full
SO  - Anticancer Res2016 Sep 01; 36
AB  - Background/Aim: We developed a novel platform to produce fully human antibodies that could be used as tools in cancer research. This study was based on maturation of B-cells into plasma cells and the subsequent secretion of fully human antibodies against a 9-mer peptide. Materials and Methods: A peptide was introduced to dendritic cells, followed by co-cultivation with cluster of differentiation 4 (CD4+) cells from healthy donors and CD19+ cells from patients with cancer. In order to detect plasma cells, we used microscopy, flow cytometry, enzyme-linked immunosorbent assays (ELISAs), and western blotting. Results. Oval-shaped cells with eccentric nuclei were found which expressed high levels of CD27 (up to 73%) and CD138 (up to 31%). An antibody against the selected peptide was detected in the culture supernatants of all samples according to ELISA and western blot analyses. Conclusion: This platform successfully generated fully human antibodies against a specific epitope.