RT Journal Article SR Electronic T1 Reduction in Proportion of Senescent CD8+ T Lymphocytes During Chemotherapy of Children with Acute Lymphoblastic Leukemia JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6195 OP 6199 VO 36 IS 11 A1 VILMA RUTKAUSKAITĖ A1 LINA RAGELIENĖ A1 RĖDA MATUZEVIČIENĖ A1 KATAŽINA SINKEVIČ A1 MYKOLAS MAURICAS A1 DAINIUS CHARACIEJUS YR 2016 UL http://ar.iiarjournals.org/content/36/11/6195.abstract AB Aim: To evaluate quantitative changes in B, NK and T lymphocyte subsets in peripheral blood of children with acute lymphoblastic leukemia (ALL) undergoing chemotherapy. Patients and Methods: Children with ALL were treated according to NOPHO ALL 2008 protocol. Levels of B lymphocytes (CD19+), NK cells (CD3−CD56+) and subsets of T lymphocytes (CD3+CD4+, CD4+CD25+Foxp3+, CD3+CD8+, CD3+CD8+CD57+, CD3+CD8+CD57−) in peripheral blood were analyzed by flow cytometry prior and during treatment with cytotoxic drugs. Results: Immunological analyses were performed in 25 children with ALL. Levels of B and NK lymphocytes decreased continuously during chemotherapy. In contrast, levels of most T lymphocyte subsets decreased only transiently and returned to pretreatment levels by days 78 to 85. The only T lymphocyte subset that did not return to the pretreatment level contained senescent CD3+CD8+CD57+ lymphocytes. Conclusion: Immunomodulating action of chemotherapy in children with ALL results in reduction of proportion of senescent CD8+ T lymphocytes.