RT Journal Article
SR Electronic
T1 Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4601
OP 4606
VO 36
IS 9
A1 ATANASOVA, SEVERINA
A1 NIKOLOVA, BILIANA
A1 MURAYAMA, SHUHEI
A1 STOYANOVA, ELENA
A1 TSONEVA, IANA
A1 ZHELEV, ZHIVKO
A1 AOKI, ICHIO
A1 BAKALOVA, RUMIANA
YR 2016
UL http://ar.iiarjournals.org/content/36/9/4601.abstract
AB Background: Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability. Materials and Methods: The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test. Results: Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction. Conclusion: The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine.