PT  - JOURNAL ARTICLE
AU  - ATANASOVA, SEVERINA
AU  - NIKOLOVA, BILIANA
AU  - MURAYAMA, SHUHEI
AU  - STOYANOVA, ELENA
AU  - TSONEVA, IANA
AU  - ZHELEV, ZHIVKO
AU  - AOKI, ICHIO
AU  - BAKALOVA, RUMIANA
TI  - Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System
DP  - 2016 Sep 01
TA  - Anticancer Research
PG  - 4601--4606
VI  - 36
IP  - 9
4099  - http://ar.iiarjournals.org/content/36/9/4601.short
4100  - http://ar.iiarjournals.org/content/36/9/4601.full
SO  - Anticancer Res2016 Sep 01; 36
AB  - Background: Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability. Materials and Methods: The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test. Results: Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction. Conclusion: The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine.