TY - JOUR T1 - Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System JF - Anticancer Research JO - Anticancer Res SP - 4601 LP - 4606 VL - 36 IS - 9 AU - SEVERINA ATANASOVA AU - BILIANA NIKOLOVA AU - SHUHEI MURAYAMA AU - ELENA STOYANOVA AU - IANA TSONEVA AU - ZHIVKO ZHELEV AU - ICHIO AOKI AU - RUMIANA BAKALOVA Y1 - 2016/09/01 UR - http://ar.iiarjournals.org/content/36/9/4601.abstract N2 - Background: Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability. Materials and Methods: The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test. Results: Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction. Conclusion: The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine. ER -