RT Journal Article
SR Electronic
T1 Photodynamic Diagnosis of Hepatocellular Carcinoma Using 5-Aminolevulinic Acid
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4569
OP 4574
VO 36
IS 9
A1 NISHIMURA, MASUMI
A1 MURAYAMA, YASUTOSHI
A1 HARADA, KYOICHI
A1 KAMADA, YOSUKE
A1 MORIMURA, RYO
A1 IKOMA, HISASHI
A1 ICHIKAWA, DAISUKE
A1 FUJIWARA, HITOSHI
A1 OKAMOTO, KAZUMA
A1 OTSUJI, EIGO
YR 2016
UL http://ar.iiarjournals.org/content/36/9/4569.abstract
AB Backgtound/Aim: Since hepatocellular carcinoma (HCC) has a high recurrence rate, accurate diagnosis of its location and curative resection is important to improve survival. This study evaluated the utility of photodynamic diagnosis (PDD) using 5-aminolevulinic acid (5-ALA) for HCC. Materials and Methods: We used two human hepatoma cell lines (HuH-7 and Hep G2). Cells were treated with 5-ALA for 4 h. 5-ALA-induced fluorescence was then examined under a fluorescence microscope. We designed hepatoma mouse models, with mice receiving an intraperitoneal injection of 5-ALA. After 4 h, their liver tumors were removed and examined under a fluorescence microscope. We also analyzed 12 HCC patients who underwent curative liver resection. The patients were administered 5-ALA orally before surgery. The excised livers were sectioned and examined by fluorescence microscopy. Results: In vitro and in vivo, red fluorescence of protoporphyrin IX (PpIX) was observed in tumors. In 11 of 12 patients, red fluorescence was observed in their HCC. The tumor of only one patient did not exhibit red fluorescence because it had been necrosed by transcatheter arterial chemoembolization (TACE). Conclusion: Red fluorescence of PpIX was observed in hepatoma cells, tumors of HCC mouse models and HCC of patients. PDD of HCC using 5-ALA is simple and may be useful for real-time diagnosis during liver resection.