PT  - JOURNAL ARTICLE
AU  - ASHLEY C. MAYS
AU  - JEFF CHOU
AU  - ANN L. CRADDOCK
AU  - LANCE MILLER
AU  - JAMES D. BROWNE
TI  - Gene Variability Between Perineural-positive and Perineural-negative Squamous Cell Skin Cancers
DP  - 2016 Aug 01
TA  - Anticancer Research
PG  - 4007--4011
VI  - 36
IP  - 8
4099  - http://ar.iiarjournals.org/content/36/8/4007.short
4100  - http://ar.iiarjournals.org/content/36/8/4007.full
SO  - Anticancer Res2016 Aug 01; 36
AB  - Aim: To identify differentially expressed genes (DEGs) between perineural invasion-positive (PP) and -negative (PN) cutaneous squamous cell cancers (CSCC). Materials/Methods: Forty CSCC samples with and without perineural invasion were processed for RNA isolation and hybridization to Affymetrix-U219 DNA microarrays. Raw gene expression data were normalized by Robust Multi-array Averaging (RMA) and log2 transformed. Gene expression-based classification models were created and accuracies evaluated using leave-one-out cross-validation. Results: At a stringent limma p-value (p<0.001), 24 genes were differentially expressed between PP and PN samples. The cross-validated performance of the eight classification models exhibited a mean accuracy of 85-95%. Diagonal linear discriminant was most accurate at 95%, followed by Bayesian compound covariate at 94%. The poorest accuracy (85%) was observed for 1-Nearest neighbor and Support vector machines. Conclusion: Gene expression may distinguish between PP and PN CSCC. Understanding these gene patterns may potentiate more timely diagnosis of perineural invasion and guide comprehensive therapies.