PT - JOURNAL ARTICLE AU - HIROYUKI INAGAWA AU - TOSHIYUKI SAIKA AU - TAKASHI NISIZAWA AU - CHIE KOHCHI AU - MAYA UENOBE AU - GEN-ICHIRO SOMA TI - Dewaxed Brown Rice Contains a Significant Amount of Lipopolysaccharide Pointing to Macrophage Activation <em>via</em> TLRs DP - 2016 Jul 01 TA - Anticancer Research PG - 3599--3605 VI - 36 IP - 7 4099 - http://ar.iiarjournals.org/content/36/7/3599.short 4100 - http://ar.iiarjournals.org/content/36/7/3599.full SO - Anticancer Res2016 Jul 01; 36 AB - Background/Aim: Oral ingestion of lipopolysaccharide (LPS) has been shown to be effective in diseases' prevention. Brown rice contains large amounts of LPS not actively consumed because of bad taste. Recently, a new type of brown rice with its wax layer removed has been produced. In this report, we measured the LPS content of this dewaxed rice and evaluated the function of innate immune activation on macrophages. Materials and Methods: Dewaxed brown rice and polished rice were prepared using the Saika-style rice polishing process. LPS content extracted using hot water from this sample was evaluated by the Limulus reaction and the activation of macrophage RAW246.7 cells was evaluated by nitric oxide (NO) production. In addition, toll-like receptors (TLRs) 2-, 4- and 9-induced human embryonic kidney (HEK) 293 cells were used for the confirmation of the activated pathway. Results: Mean LPS content in the 15 types of dewaxed brown rice was found to be 6.4±2.6 μg/g, while that of brown rice was 10.9±4.3 μg/g. The extract of dewaxed brown rice induced significant amounts of NO by RAW246.7 cells, while production was reduced to 1/6 by adding polymyxin B. The macrophage activating effect of dewaxed brown rice was 79- and 51-times higher than that of polished rice in TLR4- and 2-induced HEK 293 cells. Conclusion: LPS content in dewaxed brown rice was found to be able to activate macrophages. This rice activated macrophages mainly via the TLR4 and, to a lesser extent, TLR2 pathways. It is suggested that dewaxed brown rice can be considered effective in allergy and cancer prevention.