TY - JOUR T1 - Immunohistochemical Analysis of WT1 Antigen Expression in Various Solid Cancer Cells JF - Anticancer Research JO - Anticancer Res SP - 3715 LP - 3724 VL - 36 IS - 7 AU - KEIKO NAITOH AU - TAKASHI KAMIGAKI AU - ERIKO MATSUDA AU - HIROSHI IBE AU - SACHIKO OKADA AU - ERI OGUMA AU - YOSHIHIRO KINOSHITA AU - RISHU TAKIMOTO AU - KAORI MAKITA AU - SHUN OGASAWARA AU - SHIGENORI GOTO Y1 - 2016/07/01 UR - http://ar.iiarjournals.org/content/36/7/3715.abstract N2 - For a peptide-pulsed dendritic cell (DC) vaccine to work effectively in cancer treatment, it is significant that the target protein is expressed in cancer cells. Wilms' tumor 1 (WT1) has been identified as a molecular target for immune cell therapy of cancer. We evaluated the protein expression levels of WT1 in various solid tumors, as well as mucin 1 (MUC1) or major histocompatibility complex (MHC) class l molecules. Seven hundred and thirty-eight patients whose tissue samples were examined by immunohistochemical analysis agreed to undergo DC vaccine therapy. The positive staining of WT1 in tumor cells was observed in 25.3% of patients, with only 8.5% of them showing moderate to strong expression; moreover, WT1 tended to localize in the nucleus and cytoplasm. A positive staining of tumor cells by an anti-MHC class l monoclonal antibody was observed in 98.6% and by an anti-MUC1 monoclonal antibody in 76.8% of the patients. In relation to the application of cancer-specific immunotherapy, these findings provide useful information for determining the efficacy of MUC1- and WT1-targeted therapy. ER -