RT Journal Article
SR Electronic
T1 Development of an Evaluation Device for Phagocytic Activity of New Phagocytes Using Simple and pH-sensitive Particles that Do Not Require Pre-treatment
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3613
OP 3618
VO 36
IS 7
A1 RAN ZHANG
A1 YUTARO KOBAYASHI
A1 KIMIKO KAZUMURA
A1 HIROSHI TSUCHIYA
A1 NAOKAZU MORISHITA
A1 HIROYUKI INAGAWA
A1 GEN-ICHIRO SOMA
YR 2016
UL http://ar.iiarjournals.org/content/36/7/3613.abstract
AB Background/Aim: Phagocytic activity is affected by a number of different stress and age-dependent factors. An easy measurement of phagocytic activity is thought to allow an indicator of an individual's health. In this study, we investigated conditions of measurement to easily evaluate the activity of phagocytosis of phagocytic cells (macrophages and neutrophils) using an easy-to-use prototype, which was improved from the device by Hamamatsu Photonics K.K., to detect neutrophil activity using subtle fluorescence. Materials and Methods: pH-sensitive fluorescent particles (pHrodo-Green E. coli Bio particles, GE particles) were added to mouse-derived macrophage cell lines (J774.1) and then incubated for 2 h at 37°C. For negative control, the phagocytosis inhibitor cytochalasin D (CyD), was added prior to culture. Next, fluorescence intensity was measured by the Prototype to evaluate the phagocytic activity of macrophages and neutrophils. Phagocytosis was also confirmed by flow cytometry. Results: The Prototype detected a steady fluorescence increase in 5 sec in J774.1 after phagocytosis, using GE particles as a negative control in the presence of CyD. Furthermore, detection was possible at 104 cells/test, a concentration where the flow cytometer had difficulty for detection. Conclusion: The Prototype enables measurement of the phagocytic activity within a short period of time, even with a small sample amount, thus establishing the basic conditions of measurements of phagocytosis.