RT Journal Article
SR Electronic
T1 bFGF Up-regulation Reduces Spontaneous Necrosis of VX2 Tumors Without Increasing Tumoral Microvascular Density
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3315
OP 3320
VO 36
IS 7
A1 PASCALE, FLORENTINA
A1 GHEGEDIBAN, SAIDA-HOMAYRA
A1 BEDOUET, LAURENT
A1 NAMUR, JULIEN
A1 BONNEAU, MICHEL
A1 VERRET, VALENTIN
A1 SCHWARTZ-CORNIL, ISABELLE
A1 WASSEF, MICHEL
A1 LAURENT, ALEX
YR 2016
UL http://ar.iiarjournals.org/content/36/7/3315.abstract
AB Aim: To determine whether up-regulation of basic fibroblast growth factor (bFGF) in VX2 cells reduces tumor necrosis. Materials and Methods: VX2 cells were transfected with expression vector containing cDNA of rabbit bFGF. Stable clones producing rabbit bFGF (bFGF-VX2) were selected. bFGF-VX2 (n=5) or non-transfected VX2 (control) (n=5) cells were implanted into leg muscle of 10 rabbits. The tumors were characterized 21 days after grafting. Results: Overexpression of bFGF by VX2 tumors significantly reduced necrosis (p<0.0223) and increased cell viability (p<0.0223), without effect on the mean vascular density. bFGF concentration was significantly higher in bFGF-VX2 tumors (p<0.0062) and negatively correlated with tumor volume at day 21 (ρ=−0.927, p<0.0034). Vascular endothelial growth factor concentration was significantly lower in bFGF-VX2 tumors (p<0.0105) and negatively correlated with the bFGF concentration of tumors (ρ=−0.903, p<0.0067). Conclusion: The overexpression of bFGF in VX2 cells increased tumor viability and reduced necrosis, making the evaluation of long-term anticancer therapies possible in this model.