<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">SHIMAMURA, YOSHIKI</style></author><author><style face="normal" font="default" size="100%">TAMATANI, DAI</style></author><author><style face="normal" font="default" size="100%">KUNIYASU, SYOTA</style></author><author><style face="normal" font="default" size="100%">MIZUKI, YUSUKE</style></author><author><style face="normal" font="default" size="100%">SUZUKI, TAKUMA</style></author><author><style face="normal" font="default" size="100%">KATSURA, HANAYO</style></author><author><style face="normal" font="default" size="100%">YAMADA, HISATSUGU</style></author><author><style face="normal" font="default" size="100%">ENDO, YOSHIO</style></author><author><style face="normal" font="default" size="100%">OSAKI, TOMOHIRO</style></author><author><style face="normal" font="default" size="100%">ISHIZUKA, MASAHIRO</style></author><author><style face="normal" font="default" size="100%">TANAKA, TOHRU</style></author><author><style face="normal" font="default" size="100%">YAMANAKA, NOBUYASU</style></author><author><style face="normal" font="default" size="100%">KURAHASHI, TSUKASA</style></author><author><style face="normal" font="default" size="100%">UTO, YOSHIHIRO</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">5-Aminolevulinic Acid Enhances Ultrasound-mediated Antitumor Activity &lt;em&gt;via&lt;/em&gt; Mitochondrial Oxidative Damage in Breast Cancer</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2016-07-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">3607-3612</style></pages><volume><style face="normal" font="default" size="100%">36</style></volume><issue><style face="normal" font="default" size="100%">7</style></issue><abstract><style  face="normal" font="default" size="100%">Background/Aim: 5-Aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX), is now used for photodynamic therapy (PDT) of pre-cancers of the skin and photodynamic diagnosis (PDD) of brain tumors. Sonodynamic therapy (SDT) of cancers with ultrasound has been studied using 5-ALA as a sonosensitizer. In this article, we evaluated the sonosensitizing activity and mode of action of 5-ALA/PpIX by using mouse mammary tumor EMT6 cells. Results: 5-ALA-SDT showed significant antitumor effects toward EMT6 cells in vitro and in vivo. The fluorescence of MitoSOX Red, an indicator specific for mitochondrial superoxide, was significantly increased by 5-ALA-SDT. Moreover, the fluorescence derived from JC-1, an indicator of mitochondrial membrane potential, was also significantly increased by 5-ALA-SDT. These findings suggest that mitochondria are one of the target organelles of 5-ALA-SDT. PpIX enhanced reactive oxygen species (ROS) production from tert-butyl hydroperoxide (tBHP), suggesting that PpIX might stabilize or promote ROS generation from tBHP. Conclusion: 5-ALA-SDT showed an antitumor effect in mouse mammary tumor EMT6 cells through oxidation of the mitochondrial membrane via ROS production.</style></abstract></record></records></xml>