RT Journal Article
SR Electronic
T1 Growth Inhibition of Osteosarcoma Cell Lines in 3D Cultures: Role of Nitrosative and Oxidative Stress
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 221
OP 229
VO 36
IS 1
A1 GORSKA, MAGDALENA
A1 KRZYWIEC, PAWEL BIENIASZ
A1 KUBAN-JANKOWSKA, ALICJA
A1 ZMIJEWSKI, MICHAL
A1 WOZNIAK, MICHAL
A1 WIERZBICKA, JUSTYNA
A1 PIOTROWSKA, ANNA
A1 SIWICKA, KAROLINA
YR 2016
UL http://ar.iiarjournals.org/content/36/1/221.abstract
AB Background: 3D cell cultures have revolutionized the understanding of cell behavior, allowing culture of cells with the possibility of resembling in vivo intercellular signaling and cell–extracellular matrix interaction. Aim: The effect of limited oxygen penetration into 3D culture of highly metastatic osteosarcoma 143B cells in terms of expression of nitro-oxidative stress markers was investigated and compared to standard 2D cell culture. Materials and Methods: Human osteosarcoma (143B cell line) cells were cultured as monolayers, in collagen and Matrigel. Cell viability, gene expression of nitro-oxidative stress markers, and vascular endothelial growth factor were determined using Trypan blue assay, quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: Three-dimensional environments modify nitro-oxidative stress and influence gene expression and cell proliferation of OS 143B cells. Conclusion: Commercial cell lines might not constitute a good model of 3D cultures for bone tissue engineering, as they are highly sensitive to hypoxia, and hypoxic conditions can induce oxidation of the cellular environment.