TY - JOUR T1 - Growth Inhibition of Osteosarcoma Cell Lines in 3D Cultures: Role of Nitrosative and Oxidative Stress JF - Anticancer Research JO - Anticancer Res SP - 221 LP - 229 VL - 36 IS - 1 AU - MAGDALENA GORSKA AU - PAWEL BIENIASZ KRZYWIEC AU - ALICJA KUBAN-JANKOWSKA AU - MICHAL ZMIJEWSKI AU - MICHAL WOZNIAK AU - JUSTYNA WIERZBICKA AU - ANNA PIOTROWSKA AU - KAROLINA SIWICKA Y1 - 2016/01/01 UR - http://ar.iiarjournals.org/content/36/1/221.abstract N2 - Background: 3D cell cultures have revolutionized the understanding of cell behavior, allowing culture of cells with the possibility of resembling in vivo intercellular signaling and cell–extracellular matrix interaction. Aim: The effect of limited oxygen penetration into 3D culture of highly metastatic osteosarcoma 143B cells in terms of expression of nitro-oxidative stress markers was investigated and compared to standard 2D cell culture. Materials and Methods: Human osteosarcoma (143B cell line) cells were cultured as monolayers, in collagen and Matrigel. Cell viability, gene expression of nitro-oxidative stress markers, and vascular endothelial growth factor were determined using Trypan blue assay, quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: Three-dimensional environments modify nitro-oxidative stress and influence gene expression and cell proliferation of OS 143B cells. Conclusion: Commercial cell lines might not constitute a good model of 3D cultures for bone tissue engineering, as they are highly sensitive to hypoxia, and hypoxic conditions can induce oxidation of the cellular environment. ER -