PT  - JOURNAL ARTICLE
AU  - JERZY WIŚNIEWSKI
AU  - OLGA WESOŁOWSKA
AU  - KAMILA ŚRODA-POMIANEK
AU  - MARIA PAPROCKA
AU  - ALEKSANDRA BIELAWSKA-POHL
AU  - AGNIESZKA KRAWCZENKO
AU  - NOELIA DUARTE
AU  - MARIA-JOSÉ U. FERREIRA
AU  - DANUTA DUŚ
AU  - KRYSTYNA MICHALAK
TI  - <em>Euphorbia</em> Species-derived Diterpenes and Coumarins as Multidrug Resistance Modulators in Human Colon Carcinoma Cells
DP  - 2016 May 01
TA  - Anticancer Research
PG  - 2259--2264
VI  - 36
IP  - 5
4099  - http://ar.iiarjournals.org/content/36/5/2259.short
4100  - http://ar.iiarjournals.org/content/36/5/2259.full
SO  - Anticancer Res2016 May 01; 36
AB  - Background: Recently, many new potent multidrug resistance (MDR) reversal agents have been discovered, among them lathyrane and jatrophane diterpenes isolated from various Euphorbia species. In the present study, the cytotoxicity, P-glycoprotein inhibition activity, and MDR reversal potency of six diterpenes and two coumarins from two Euphorbia species were studied in human colon carcinoma LoVo cells, and doxorubicin-resistant, LoVo/Dx cells. Materials and Methods: Cytotoxicity of the studied compounds (alone and in combination with doxorubicin) was investigated. Inhibition of P-glycoprotein transport activity was monitored by flow cytometry. Changes in intracellular doxorubicin accumulation were observed by means of fluorescence microscopy. Results: Latilagascene B was demonstrated to be an effective P-glycoprotein inhibitor, able to increase doxorubicin accumulation in resistant cells, however not able to restore doxorubicin cytotoxicity in LoVo/Dx cells. Conclusion: The structure of latilagascene B seems to be an interesting candidate for further synthesis of new derivatives of reduced cytotoxicity and high anti-MDR potency.