RT Journal Article SR Electronic T1 PIK3CA Mutations Detected in Patients with Central Nervous System Metastases of Non-small Cell Lung Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2243 OP 2249 VO 36 IS 5 A1 MARCIN NICOŚ A1 PAWEŁ KRAWCZYK A1 TOMASZ POWRÓZEK A1 PAULINA SZUDY A1 BOŻENA JAROSZ A1 MAREK SAWICKI A1 JUSTYNA SZUMIŁO A1 TOMASZ TROJANOWSKI A1 JANUSZ MILANOWSKI YR 2016 UL http://ar.iiarjournals.org/content/36/5/2243.abstract AB Background: In non-small cell lung cancer (NSCLC) the phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) gene mutations have been reported in fewer than 5% of primary tumors. Materials and Methods: We assessed PIK3CA gene mutations in 145 tissue samples from central nervous system (CNS) metastases of NSCLC using three polymerase chain reaction (PCR) techniques: high resolution melting-PCR (HRM-PCR), allele-specific-quantitative PCR (ASP-qPCR) and TaqMan PCR. Results: HRM analysis allowed us to select three PIK3CA-positive specimens (2.1% of the studied group) and ASP-qPCR techniques identified them as one E542K and two H1047R substitutions, which were confirmed by TaqMan probes. The PIK3CA mutations were indicated only in males (3% of all males). One of the patients was reported to be a non-smoker with adenocarcinoma (AC; 2.5% of the AC group), however, the other two patients were smokers with squamous cell carcinoma (SCC; 3.4% of SCC group). Conclusion: This is the first report of the presence of PIK3CA gene mutation in CNS-metastatic lesions of NSCLC worldwide that could broaden therapeutic choices in such patients.