RT Journal Article
SR Electronic
T1 Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1507
OP 1518
VO 36
IS 4
A1 FLORIAN GEBAUER
A1 DANIEL WICKLEIN
A1 MICHAEL TACHEZY
A1 TOBIAS GROB
A1 DORIS STEINEMANN
A1 GEORGI MANUKJAN
A1 GUDRUN GÖHRING
A1 BRIGITTE SCHLEGELBERGER
A1 HANNA MAAR
A1 JAKOB R. IZBICKI
A1 MAXIMILIAN BOCKHORN
A1 UDO SCHUMACHER
YR 2016
UL http://ar.iiarjournals.org/content/36/4/1507.abstract
AB Background: Non-small lung cancer is the leading cause of cancer-related mortality worldwide. For a deeper understanding of tumor biology, we established a pair of cell lines derived from a primary tumor and a corresponding lymph node metastasis. Material and Methods: The cell line BC4323 from the primary tumor (PT) and a mediastinal lymph node metastasis (LN) were derived from an adenocarcinoma (pT2, pN2, G3, UICC stage IIIa) in a 47-year-old female patient. Comparative characterization was performed by in vitro analysis. A murine xenograft was established for analysis of in vivo behavior. Results: Chromosomal aberrations were detected in multiple chromosomal sections throughout the entire genome, with only a few differences between PT and LN cells. High-level Kirsten ras oncogene homolog (KRAS) mutation and amplification were seen based on a chromosomal translocation and novel assembled chromosome. In contrast to the genomic level, at the mRNA and protein levels, multiple differences were detectable, in particular in markers for cell adhesion [e.g. epithelial cell adhesion molecule (EpCAM), CD44, P-selectin binding, epidermal growth factor receptor (EGFR) and integrin alphaV] and the epithelial–mesenchymal transition. Due to accelerated tumor growth in vivo by the PT cells, a shortened overall survival was seen (60 vs. 101 days, p=0.005). Conclusion: We provide detailed analysis of a cell line derived from a primary tumor and a corresponding LN metastasis. This unique feature allows further investigative analysis of the differences and regulatory processes underlying the metastatic process during tumor progression in non-small cell lung cancer.