PT  - JOURNAL ARTICLE
AU  - AKIRA KUMASAKA
AU  - NAOYUKI MATSUMOTO
AU  - SHOTARO MUKAE
AU  - TAIICHI KITANO
AU  - HIROYASU NOGUCHI
AU  - HIDERO OHKI
AU  - KAZUO KOMIYAMA
AU  - TOMOHIRO ANDO
TI  - Rapid and Specific Screening Assay for <em>KRAS</em> Oncogene Mutation by a Novel Gene Amplification Method
DP  - 2016 Apr 01
TA  - Anticancer Research
PG  - 1571--1579
VI  - 36
IP  - 4
4099  - http://ar.iiarjournals.org/content/36/4/1571.short
4100  - http://ar.iiarjournals.org/content/36/4/1571.full
SO  - Anticancer Res2016 Apr 01; 36
AB  - Background: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making. Materials and Methods: KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources. Conclusion: This newly developed procedure can be useful for patient stratification for anti-EGFR therapies.