RT Journal Article
SR Electronic
T1 Inhibition of Survivin Is Associated with Zoledronic Acid-induced Apoptosis of Prostate Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 913
OP 920
VO 36
IS 3
A1 MARTINA FRAGNI
A1 SARA ANNA BONINI
A1 ARMANDO STABILE
A1 SERENA BODEI
A1 LUCA CRISTINELLI
A1 CLAUDIO SIMEONE
A1 DANILO ZANI
A1 PIER FRANCO SPANO
A1 ALFREDO BERRUTI
A1 MAURIZIO MEMO
A1 SANDRA SIGALA
YR 2016
UL http://ar.iiarjournals.org/content/36/3/913.abstract
AB Background/Aim: Evidence suggests that zoledronic acid (ZA) exerts direct antitumor effects on cancer cells but the underlying mechanisms of these actions are unknown. This study investigated the possible involvement of survivin in the antiproliferative effects of ZA in prostate cancer. Materials and Methods: 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye reduction assay was used to assess cell viability and acridine orange/ethidium bromide double staining to analyze cell death. Human Apoptosis Array evaluated the expression of apoptosis-related proteins. Survivin protein was measured by western blot technique and miR-203 levels were quantified by quantitative real-time polymerase chain reaction. Results: ZA induced inhibition of cell proliferation and apoptosis activation, with down-regulation of survivin protein. A negative regulation at gene expression level may be hypothesized because we observed a significant decrease of survivin mRNA level and an increase of miR-203 expression after ZA exposure. Conclusion: This study provides evidence that ZA may directly inhibit cancer cell proliferation, identifying survivin as one of its downstream targets.