TY - JOUR T1 - Expression of Semaphorin 3C in Breast Cancer and its Impact on Adhesion and Invasion of Breast Cancer Cells JF - Anticancer Research JO - Anticancer Res SP - 1281 LP - 1286 VL - 36 IS - 3 AU - MUHAMMAD FARAZ ARSHAD MALIK AU - LUCY K. SATHERLEY AU - ELERI L. DAVIES AU - LIN YE AU - WEN G. JIANG Y1 - 2016/03/01 UR - http://ar.iiarjournals.org/content/36/3/1281.abstract N2 - Background: The aim of the current study was to examine the role of semaphorin 3C (SEMA3C) in breast cancer. Materials and Methods: SEMA3C transcripts expressed by breast tissues were determined using real-time polymerase chain reaction (PCR). Knock-down of SEMA3C was performed in MDA-MB-231 and MCF-7 breast cancer cell lines using anti-SEMA3C hammerhead ribozyme transgenes. The effect of SEMA3C knockdown on cancer cells was determined using in vitro cellular function assays. Results: Higher SEMA3C transcript levels were significantly associated with poor differentiation of cancer cells, and transcript levels were significantly reduced in oestrogen receptor-positive tumours. Knock-down of SEMA3C expression resulted in a decrease in cell proliferation, adhesion and invasion of breast cancer cells. Conclusion: Higher SEMA3C expression is correlated with tumour differentiation. Inhibition of SEMA3C reduces adhesion and invasion of breast cancer cells. This suggests that SEMA3C may play a significant role in morphological changes of cancer cells, leading to enhanced growth and dissemination. ER -