@article {WEEKS1135, author = {HOI PING WEEKS and ANDREW J. SANDERS and HOWARD G. KYNASTON and WEN G. JIANG}, title = {The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells}, volume = {36}, number = {3}, pages = {1135--1142}, year = {2016}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Actin polymerisation is stimulated by the actin-related protein (ARP) 2/3 complex and drives cell migration. This complex is activated by Wiskott-Aldrich syndrome protein family (WASP) verprolin homologous protein (WAVE) proteins. WAVE1 and -3 have been implicated in the aggressiveness of metastatic prostate cancer cells. Materials and Methods: Cell growth, motility and invasion were analyzed in WAVE1- and WAVE3-knockdown PC-3 cells along with the ARP2/3 inhibitor, CK-0944636. Confocal microscopy was adopted to examine protein co-localisation. Immunoprecipitation approaches were used to determine protein tyrosine phosphorylation. Results: Cell growth suppression was observed with WAVE3 knockdown and ARP2/3 inhibition. Reduced cell invasion effects observed with WAVE1 knockdown appeared to be rescued by ARP2/3 inhibition. WAVE1 and WAVE3 and ARP2 co-localisation was lost in PC-3 WAVE-knockdown cells, while increased ARP2 tyrosine phosphorylation was observed with WAVE3 knockdown. Conclusion: These results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/36/3/1135}, eprint = {https://ar.iiarjournals.org/content/36/3/1135.full.pdf}, journal = {Anticancer Research} }