TY - JOUR T1 - Microarray Analysis of Gene Expression at the Tumor Front of Colon Cancer JF - Anticancer Research JO - Anticancer Res SP - 6577 LP - 6581 VL - 35 IS - 12 AU - TAKAAKI KOBAYASHI AU - TADAHIKO MASAKI AU - ERIKO NOZAKI AU - MASANORI SUGIYAMA AU - FUMIO NAGASHIMA AU - JUNJI FURUSE AU - HIROAKI ONISHI AU - TAKASHI WATANABE AU - YASUO OHKURA Y1 - 2015/12/01 UR - http://ar.iiarjournals.org/content/35/12/6577.abstract N2 - Budding or the presence poorly differentiated clusters at the boundary of cancer tissue is a pathologically important finding and serves as a prognostic factor in colorectal cancer. However, few studies have examined the cancer tissue boundary in clinical samples. The purpose of the present study was to examine gene expression at the tumor front of colon cancer in surgically resected samples. Cancer tissues were obtained by laser microdissection of 20 surgically resected specimens. Genes with significantly different microarray signals between the tumor front and the tumor center were identified. Among genes showing significant up-regulation at the tumor front were six chemokines [chemokine c-c motif ligand (CCL)2 and -18, chemokine (C-X-C motif) ligand (CXCL)9-11, and interleukin 8 (IL8)], and two apoptosis-related molecules [ubiquitin D (UBD) and baculoviral iap repeat-containing 3 (BIRC3)]. Expression of laminin gamma 2 (LAMC2), matrix metallopeptidase 7 (MMP7) and epithelial–mesenchymal transition (EMT)-related molecules were elevated in the tumor front, but their fold changes were smaller than those of the aforementioned genes. These results suggest that chemokines, in addition to EMT-related molecules, may play important roles in invasion of colon cancer. ER -