RT Journal Article SR Electronic T1 Breast Cancer Cell Lines Exhibit Differential Sensitivities to Microtubule-targeting Drugs Independent of Doubling Time JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 5845 OP 5850 VO 35 IS 11 A1 APRIL L. RISINGER A1 NICHOLAS F. DYBDAL-HARGREAVES A1 SUSAN L. MOOBERRY YR 2015 UL http://ar.iiarjournals.org/content/35/11/5845.abstract AB Background: Microtubule-targeting agents (MTAs) are a mainstay in breast cancer treatment, yet patient responses differ. The underlying mechanisms of these differences are unknown. While MTAs are mitotic inhibitors, recent evidence highlights that non-mitotic effects of these drugs can contribute to their anticancer effects. It is critical to identify the non-mitotic mechanisms that could contribute to differences among MTAs. However, it is not clear whether rapidly dividing cells in culture are optimal tools to address these mechanistic questions in interphase cells. Materials and Methods: Detailed concentration response curves for five MTAs in a panel of diverse breast cancer cell lines were generated. Results: Substantial differences among both drugs and cell lines, consistent with the clinical scenario, were observed. Importantly, these differences do not correlate with cell doubling time. Conclusion: The interphase actions of MTAs are critical to the full spectrum of their effects in cancer cells, even in cell culture models.