@article {JING5839, author = {YONGBIN JING and HONGBIN LIANG and YIFAN ZHANG and JOSEPH CLEVELAND and JINGLONG YAN and DEKAI ZHANG}, title = {Up-regulation of Toll-like Receptor 9 in Osteosarcoma}, volume = {35}, number = {11}, pages = {5839--5843}, year = {2015}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Increasing evidence has shown that Toll-like receptors (TLRs), key receptors in innate immunity, play a role in cancer development and progression. The present study aimed to elucidate the role of TLR expression in osteosarcoma cancer cells and patient specimens. Materials and Methods: We investigated the expression of all of human TLRs in osteosarcoma MG-63 cells by real-time quantitative reverse transcription polymerase chain reaction. We then further explored whether the up-regulation of TLR9 expression is common in patients with osteosarcoma by examining TLR9 protein levels in 80 osteosarcoma specimens and 28 normal controls by immunohistochemistry. Results: We found that among TLR family members, TLR9 was predominately expressed in osteosarcoma cells, and up-regulation of TLR9 expression was found in 72 out of 80 (90\%) patients with osteosarcoma but in none of 28 normal controls. Furthermore, high expression of TLR9 appeared to be associated with osteosarcoma progression. Conclusion: TLR9 is up-regulated in the majority of osteosarcomas, which appears to play an important role in osteosarcoma development and progression. Therefore, TLR9 may serve as a novel therapeutic target for human osteosarcoma therapy.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/35/11/5839}, eprint = {https://ar.iiarjournals.org/content/35/11/5839.full.pdf}, journal = {Anticancer Research} }