RT Journal Article
SR Electronic
T1 Effects of Vitamin K<sub>3</sub> and K<sub>5</sub> on Daunorubicin-resistant Human T Lymphoblastoid Leukemia Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6041
OP 6048
VO 35
IS 11
A1 ERI NAKAOKA
A1 SACHIKO TANAKA
A1 KENJI ONDA
A1 KENTARO SUGIYAMA
A1 TOSHIHIKO HIRANO
YR 2015
UL http://ar.iiarjournals.org/content/35/11/6041.abstract
AB Background/Aim: Anticancer efficacy of vitamin K derivatives on multidrug-resistant cancer cells has been scarcely investigated. Materials and Methods: The effects of vitamins K3 and K5 on proliferation of human leukemia MOLT-4 cells and on daunorubicin-resistant MOLT-4/DNR cells were estimated by a WST assay. Apoptotic cells were detected by Annexin V and propidium iodide staining, followed by flow cytometry. Results: Vitamins K3 and K5 significantly inhibited proliferation of leukemic cells at 10 and 100 μM (p&lt;0.05), and these effects were almost equally observed in both MOLT-4 and MOLT/DNR drug-resistant cells. Vitamin K3 induced cell apoptosis at 10 and 100 μM in both MOLT-4 and MOLT-4/DNR cells (p&lt;0.05). Vitamin K5 also increased apoptotic cells, while rather inducing necrotic cell death. Conclusion: Vitamins K3 and K5 suppress MOLT-4 and MOLT-4/DNR cell-proliferation partially through induction of apoptosis, and these vitamin derivatives can overcome drug resistance due to P-glycoprotein expression.