PT  - JOURNAL ARTICLE
AU  - MAURO PROVINCIALI
AU  - ELISA PIERPAOLI
AU  - BEATRICE BARTOZZI
AU  - GIOVANNI BERNARDINI
TI  - Zinc Induces Apoptosis of Human Melanoma Cells, Increasing Reactive Oxygen Species, p53 and FAS Ligand
DP  - 2015 Oct 01
TA  - Anticancer Research
PG  - 5309--5316
VI  - 35
IP  - 10
4099  - http://ar.iiarjournals.org/content/35/10/5309.short
4100  - http://ar.iiarjournals.org/content/35/10/5309.full
SO  - Anticancer Res2015 Oct 01; 35
AB  - The aim of this study was to examine the in vitro effect of zinc on the apoptosis of human melanoma cells, by studying the zinc-dependent modulation of intracellular levels of reactive oxygen species (ROS) and of p53 and FAS ligand proteins. We showed that zinc concentrations ranging from 33.7 μM to 75 μM Zn2+ induced apoptosis in the human melanoma cell line WM 266-4. This apoptosis was associated with an increased production of intracellular ROS, and of p53 and FAS ligand protein. Treatment of tumor cells with the antioxidant N-acetylcysteine was able to prevent Zn2+-induced apoptosis, as well as the increase of p53 and FAS ligand protein induced by zinc. Zinc induces apoptosis in melanoma cells by increasing ROS and this effect may be mediated by the ROS-dependent induction of p53 and FAS/FAS ligand.