TY - JOUR T1 - Zinc Induces Apoptosis of Human Melanoma Cells, Increasing Reactive Oxygen Species, p53 and FAS Ligand JF - Anticancer Research JO - Anticancer Res SP - 5309 LP - 5316 VL - 35 IS - 10 AU - MAURO PROVINCIALI AU - ELISA PIERPAOLI AU - BEATRICE BARTOZZI AU - GIOVANNI BERNARDINI Y1 - 2015/10/01 UR - http://ar.iiarjournals.org/content/35/10/5309.abstract N2 - The aim of this study was to examine the in vitro effect of zinc on the apoptosis of human melanoma cells, by studying the zinc-dependent modulation of intracellular levels of reactive oxygen species (ROS) and of p53 and FAS ligand proteins. We showed that zinc concentrations ranging from 33.7 μM to 75 μM Zn2+ induced apoptosis in the human melanoma cell line WM 266-4. This apoptosis was associated with an increased production of intracellular ROS, and of p53 and FAS ligand protein. Treatment of tumor cells with the antioxidant N-acetylcysteine was able to prevent Zn2+-induced apoptosis, as well as the increase of p53 and FAS ligand protein induced by zinc. Zinc induces apoptosis in melanoma cells by increasing ROS and this effect may be mediated by the ROS-dependent induction of p53 and FAS/FAS ligand. ER -