RT Journal Article SR Electronic T1 Sensitivity of Human Malignant Melanoma Cell Lines to Newcastle Disease Virus JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 5401 OP 5406 VO 35 IS 10 A1 MARIANNA PAP A1 JUDIT BÁTOR A1 JÓZSEF SZEBERÉNYI YR 2015 UL http://ar.iiarjournals.org/content/35/10/5401.abstract AB Background/Aim: Virotherapy may be a promising alternative to chemotherapy of malignant melanoma. In clinical trials using strains of Newcastle disease virus (NDV), only a fraction of patients with cancer responded to virotherapy. In the present study, we tried to find a correlation between the susceptibility of human melanoma cell lines to NDV and growth factor signaling pathways. Materials and Methods: Using an ATP assay, cytotoxicity of an NDV strain (MTH-68/H) was tested in 13 human melanoma cell lines. The activation state of growth factor signaling pathways was studied by the analysis of key signaling proteins. Results: MTH-68/H was found to be cytotoxic in all melanoma cells tested, but the IC50 values varied significantly. No correlation between the IC50 values and the rate of extracellular signal-regulated kinase (ERK) and AKT phosphorylation and phosphatase and tensin homologue (PTEN) expression was found. Conclusion: Susceptibility of tumor cells to NDV may be affected by alterations other than those of RAS/ERK and phosphatidylinositol 3-kinase (PI3K)/AKT signaling in uninfected cells.