TY - JOUR T1 - Sensitivity of Human Malignant Melanoma Cell Lines to Newcastle Disease Virus JF - Anticancer Research JO - Anticancer Res SP - 5401 LP - 5406 VL - 35 IS - 10 AU - MARIANNA PAP AU - JUDIT BÁTOR AU - JÓZSEF SZEBERÉNYI Y1 - 2015/10/01 UR - http://ar.iiarjournals.org/content/35/10/5401.abstract N2 - Background/Aim: Virotherapy may be a promising alternative to chemotherapy of malignant melanoma. In clinical trials using strains of Newcastle disease virus (NDV), only a fraction of patients with cancer responded to virotherapy. In the present study, we tried to find a correlation between the susceptibility of human melanoma cell lines to NDV and growth factor signaling pathways. Materials and Methods: Using an ATP assay, cytotoxicity of an NDV strain (MTH-68/H) was tested in 13 human melanoma cell lines. The activation state of growth factor signaling pathways was studied by the analysis of key signaling proteins. Results: MTH-68/H was found to be cytotoxic in all melanoma cells tested, but the IC50 values varied significantly. No correlation between the IC50 values and the rate of extracellular signal-regulated kinase (ERK) and AKT phosphorylation and phosphatase and tensin homologue (PTEN) expression was found. Conclusion: Susceptibility of tumor cells to NDV may be affected by alterations other than those of RAS/ERK and phosphatidylinositol 3-kinase (PI3K)/AKT signaling in uninfected cells. ER -