TY - JOUR T1 - Pro-apoptotic and Growth-inhibitory Effect of <em>IFN</em>-β-Overexpressing Canine Adipose Tissue-derived Mesenchymal Stem Cells Against Melanoma Cells JF - Anticancer Research JO - Anticancer Res SP - 4749 LP - 4756 VL - 35 IS - 9 AU - SEI-MYOUNG HAN AU - CHONG-WOO PARK AU - JIN-OK AHN AU - SANG-CHUL PARK AU - WOO-SUNG JUNG AU - KYOUNG-WON SEO AU - JEONG-CHAN RA AU - SUNG-KEUN KANG AU - HEE-WOO LEE AU - HWA- YOUNG YOUN Y1 - 2015/09/01 UR - http://ar.iiarjournals.org/content/35/9/4749.abstract N2 - Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissue-derived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSC-conditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with pro-apoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma. ER -