PT  - JOURNAL ARTICLE
AU  - SEI-MYOUNG HAN
AU  - CHONG-WOO PARK
AU  - JIN-OK AHN
AU  - SANG-CHUL PARK
AU  - WOO-SUNG JUNG
AU  - KYOUNG-WON SEO
AU  - JEONG-CHAN RA
AU  - SUNG-KEUN KANG
AU  - HEE-WOO LEE
AU  - HWA- YOUNG YOUN
TI  - Pro-apoptotic and Growth-inhibitory Effect of <em>IFN</em>-β-Overexpressing Canine Adipose Tissue-derived Mesenchymal Stem Cells Against Melanoma Cells
DP  - 2015 Sep 01
TA  - Anticancer Research
PG  - 4749--4756
VI  - 35
IP  - 9
4099  - http://ar.iiarjournals.org/content/35/9/4749.short
4100  - http://ar.iiarjournals.org/content/35/9/4749.full
SO  - Anticancer Res2015 Sep 01; 35
AB  - Background: Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissue-derived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells. Materials and Methods: Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis. Results: Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSC-conditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media. Conclusion: IFN-β overexpression by cATMSCs was associated with pro-apoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.