TY - JOUR T1 - Innate Immunity Activated by Oral Administration of LPSp Is Phylogenetically Preserved and Developed in Broiler Chickens JF - Anticancer Research JO - Anticancer Res SP - 4461 LP - 4466 VL - 35 IS - 8 AU - TSUTOMU KOMORI AU - KEIKO SAITO AU - NORIYUKI SAWA AU - YASUHIRO SHIBASAKI AU - CHIE KOHCHI AU - GEN-ICHIRO SOMA AU - HIROYUKI INAGAWA Y1 - 2015/08/01 UR - http://ar.iiarjournals.org/content/35/8/4461.abstract N2 - Background: Oral administration of lipopolysaccharide (LPS), a major outer-membrane component of Gram-negative bacteria, has been found to prevent infection in mammals and fish. Oral administration of LPS is believed to increase phagocytic activity and promote cytokine production, thus associating it with priming. The present study aimed to elucidate the effect of oral administration of LPS in birds, which phylogenetically lie between fish and mammals, using chickens as a model. Materials and Methods: LPS derived from Pantoea agglomerans (LPSp) was added to the feed or water for oral administration to broiler chickens. For the survival assay and gene expression analysis (Genopal), LPSp was administered at a dose of 10 μg/kg of body weight (BW)/day. LPSp was administered at a dose of 0.2 μg/kg or 200 μg/kg to stimulate peripheral blood mononuclear phagocytosis (latex beads) and nitric oxide (NO) production. Results: LPSp (10 μg/kg BW/day) administration significantly inhibited mortality in broiler chickens on commercial farms. Furthermore, oral administration resulted in a transient increase in phagocytic activity and improved the ability to produce NO. On examining splenic cytokine induction following intraperitoneal administration of LPS derived from Escherichia coli (LPSe), we found significantly increased interleukin (IL)-1β mRNA expression. Conclusion: Innate immunity activation in chickens, as seen in infection prevention, was induced by oral administration of LPSp. This infection prevention involved increased phagocytic activity and enhanced gene expression and appears to be a phylogenetically-preserved innate immunity mechanism. ER -