RT Journal Article SR Electronic T1 Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4447 OP 4451 VO 35 IS 8 A1 YU SUMIYA A1 MAMI ISHIKAWA A1 TAKAHIRO INOUE A1 TOSHIO INUI A1 DAISUKE KUCHIIKE A1 KENTARO KUBO A1 YOSHIHIRO UTO A1 TAKAHITO NISHIKATA YR 2015 UL http://ar.iiarjournals.org/content/35/8/4447.abstract AB Background: Although the mechanisms of macrophage activation are important for cancer immunotherapy, they are poorly understood. Recently, easy and robust assay systems for assessing the macrophage-activating factor (MAF) using monocytic cell line-derived macrophages were established. Materials and Methods: Gene-expression profiles of U937- and THP-1-derived macrophages were compared using gene expression microarray analysis and their responses against several MAFs were examined by in vitro experiments. Results: Activated states of these macrophages could not be assigned to a specific sub-type but showed, however, different unique characteristics. Conclusion: The unique of monocytic cell line-derived macrophages could provide clues to understand the activation mechanism of macrophages and, therefore, help to develop effective cancer immunotherapy with MAFs.