RT Journal Article SR Electronic T1 Aloe vera Extract Suppresses Proliferation of Neuroblastoma Cells In Vitro JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4479 OP 4485 VO 35 IS 8 A1 AKANE YONEHARA A1 YUKA TANAKA A1 KASEM KULKEAW A1 TAKUMI ERA A1 YOICHI NAKANISHI A1 DAISUKE SUGIYAMA YR 2015 UL http://ar.iiarjournals.org/content/35/8/4479.abstract AB Background/Aim: Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. Materials and Methods: Human neuroblastoma cell lines (IMR-32, TGW, CHP-126 and NBL-S) were cultured with AV protein extract and proliferation status was assessed by cell counting, Ki-67 staining and gene expression. Results: Among tested lines, the number of viable, AV-treated IMR-32 cells significantly decreased 1.98-fold by day 2 and 1.33-fold by day 5 of culture relative to untreated controls (p<0.05). Treatment also decreased the number of Ki-67(+) IMR-32 cells by 13% by day 5 (p<0.05) and, unlike untreated controls, CCND2 mRNA expression levels became undetectable by day 1. Conclusion: AV-protein extract suppresses human IMR-32 neuroblastoma cell proliferation, possibly by suppressing CCND2 transcript levels in vitro.