RT Journal Article
SR Electronic
T1 Aloe vera Extract Suppresses Proliferation of Neuroblastoma Cells In Vitro
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4479
OP 4485
VO 35
IS 8
A1 AKANE YONEHARA
A1 YUKA TANAKA
A1 KASEM KULKEAW
A1 TAKUMI ERA
A1 YOICHI NAKANISHI
A1 DAISUKE SUGIYAMA
YR 2015
UL http://ar.iiarjournals.org/content/35/8/4479.abstract
AB Background/Aim: Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. Materials and Methods: Human neuroblastoma cell lines (IMR-32, TGW, CHP-126 and NBL-S) were cultured with AV protein extract and proliferation status was assessed by cell counting, Ki-67 staining and gene expression. Results: Among tested lines, the number of viable, AV-treated IMR-32 cells significantly decreased 1.98-fold by day 2 and 1.33-fold by day 5 of culture relative to untreated controls (p<0.05). Treatment also decreased the number of Ki-67(+) IMR-32 cells by 13% by day 5 (p<0.05) and, unlike untreated controls, CCND2 mRNA expression levels became undetectable by day 1. Conclusion: AV-protein extract suppresses human IMR-32 neuroblastoma cell proliferation, possibly by suppressing CCND2 transcript levels in vitro.