RT Journal Article
SR Electronic
T1 CD114 Expression Mediates Melanoma Tumor Cell Growth and Treatment Resistance
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3787
OP 3792
VO 35
IS 7
A1 LINNA ZHANG
A1 SAURABH AGARWAL
A1 JASON M. SHOHET
A1 PETER E. ZAGE
YR 2015
UL http://ar.iiarjournals.org/content/35/7/3787.abstract
AB Background/Aim: Melanoma tumor cell sub-populations expressing a variety of specific molecular markers have been identified. We hypothesized that expression of CD114, the cell surface receptor for granulocyte-colony stimulating factor (G-CSF), would be associated with melanoma tumor cell growth and response to treatment. Materials and Methods: We determined the expression of CD114 expression in tumor cell lines by flow cytometry. We separated melanoma tumor cells into CD114-positive and - negative populations by fluorescence-activated cell sorting (FACS) and measured cell growth and responses to temozolomide and etoposide and the anticancer agent nifurtimox. Results: All tested cell lines demonstrated a sub-population of cells with CD114 surface expression. CD114-positive sub-populations grew faster than CD114-negative ones and demonstrated resistance to temozolomide, etoposide, and nifurtimox. Conclusion: CD114 expression defines a sub-population of melanoma tumor cells with altered growth and resistance to treatment. Further studies on the role of CD114 in melanoma pathogenesis are warranted.