PT  - JOURNAL ARTICLE
AU  - LINNA ZHANG
AU  - SAURABH AGARWAL
AU  - JASON M. SHOHET
AU  - PETER E. ZAGE
TI  - CD114 Expression Mediates Melanoma Tumor Cell Growth and Treatment Resistance
DP  - 2015 Jul 01
TA  - Anticancer Research
PG  - 3787--3792
VI  - 35
IP  - 7
4099  - http://ar.iiarjournals.org/content/35/7/3787.short
4100  - http://ar.iiarjournals.org/content/35/7/3787.full
SO  - Anticancer Res2015 Jul 01; 35
AB  - Background/Aim: Melanoma tumor cell sub-populations expressing a variety of specific molecular markers have been identified. We hypothesized that expression of CD114, the cell surface receptor for granulocyte-colony stimulating factor (G-CSF), would be associated with melanoma tumor cell growth and response to treatment. Materials and Methods: We determined the expression of CD114 expression in tumor cell lines by flow cytometry. We separated melanoma tumor cells into CD114-positive and - negative populations by fluorescence-activated cell sorting (FACS) and measured cell growth and responses to temozolomide and etoposide and the anticancer agent nifurtimox. Results: All tested cell lines demonstrated a sub-population of cells with CD114 surface expression. CD114-positive sub-populations grew faster than CD114-negative ones and demonstrated resistance to temozolomide, etoposide, and nifurtimox. Conclusion: CD114 expression defines a sub-population of melanoma tumor cells with altered growth and resistance to treatment. Further studies on the role of CD114 in melanoma pathogenesis are warranted.