%0 Journal Article %A LINNA ZHANG %A SAURABH AGARWAL %A JASON M. SHOHET %A PETER E. ZAGE %T CD114 Expression Mediates Melanoma Tumor Cell Growth and Treatment Resistance %D 2015 %J Anticancer Research %P 3787-3792 %V 35 %N 7 %X Background/Aim: Melanoma tumor cell sub-populations expressing a variety of specific molecular markers have been identified. We hypothesized that expression of CD114, the cell surface receptor for granulocyte-colony stimulating factor (G-CSF), would be associated with melanoma tumor cell growth and response to treatment. Materials and Methods: We determined the expression of CD114 expression in tumor cell lines by flow cytometry. We separated melanoma tumor cells into CD114-positive and - negative populations by fluorescence-activated cell sorting (FACS) and measured cell growth and responses to temozolomide and etoposide and the anticancer agent nifurtimox. Results: All tested cell lines demonstrated a sub-population of cells with CD114 surface expression. CD114-positive sub-populations grew faster than CD114-negative ones and demonstrated resistance to temozolomide, etoposide, and nifurtimox. Conclusion: CD114 expression defines a sub-population of melanoma tumor cells with altered growth and resistance to treatment. Further studies on the role of CD114 in melanoma pathogenesis are warranted. %U https://ar.iiarjournals.org/content/anticanres/35/7/3787.full.pdf