TY - JOUR T1 - Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells JF - Anticancer Research JO - Anticancer Res SP - 3781 LP - 3786 VL - 35 IS - 7 AU - BARBARA ADINOLFI AU - SARA CARPI AU - ANTONELLA ROMANINI AU - ELEONORA DA POZZO AU - MAURA CASTAGNA AU - BARBARA COSTA AU - CLAUDIA MARTINI AU - SØREN-PETER OLESEN AU - NICOLE SCHMITT AU - MARIA CRISTINA BRESCHI AU - PAOLA NIERI AU - STEFANO FOGLI Y1 - 2015/07/01 UR - http://ar.iiarjournals.org/content/35/7/3781.abstract N2 - Aim: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells. Materials and Methods: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation. Results: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1–S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis. Conclusion: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin. ER -