TY - JOUR T1 - Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice JF - Anticancer Research JO - Anticancer Res SP - 2817 LP - 2822 VL - 35 IS - 5 AU - CELINA T.F. OSHIMA AU - DANIEL A. RIBEIRO AU - THIAGO S. GOMES AU - PRISCILA C. ADIOS AU - MIZUE I. EGAMI AU - HELENA R. C. SEGRETO Y1 - 2015/05/01 UR - http://ar.iiarjournals.org/content/35/5/2817.abstract N2 - Background/Aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. Materials and Methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami−/Rad−: Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami+/Rad−: mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami−/Rad+: mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami+/Rad+: mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. Results. In oesophagus and stomach tissues, we did not observe any difference between Ami−/ad−, Ami+/Rad−, Ami−/Rad+ and Ami+/Rad+ groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami−/Rad+group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice. ER -