RT Journal Article
SR Electronic
T1 Non-small Cell Lung Cancer Detection Using MicroRNA Expression Profiling of Bronchoalveolar Lavage Fluid and Sputum
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1873
OP 1880
VO 35
IS 4
A1 JULIAN O. KIM
A1 SAYF GAZALA
A1 RENE RAZZAK
A1 LINGHONG GUO
A1 SUNITA GHOSH
A1 WILSON H. ROA
A1 ERIC L.R. BÉDARD
YR 2015
UL http://ar.iiarjournals.org/content/35/4/1873.abstract
AB Aim: To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC). Materials and Methods: Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls. Results: Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%. Conclusion: miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC.