TY - JOUR T1 - Non-small Cell Lung Cancer Detection Using MicroRNA Expression Profiling of Bronchoalveolar Lavage Fluid and Sputum JF - Anticancer Research JO - Anticancer Res SP - 1873 LP - 1880 VL - 35 IS - 4 AU - JULIAN O. KIM AU - SAYF GAZALA AU - RENE RAZZAK AU - LINGHONG GUO AU - SUNITA GHOSH AU - WILSON H. ROA AU - ERIC L.R. BÉDARD Y1 - 2015/04/01 UR - http://ar.iiarjournals.org/content/35/4/1873.abstract N2 - Aim: To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC). Materials and Methods: Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls. Results: Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%. Conclusion: miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC. ER -