PT - JOURNAL ARTICLE AU - JULIAN O. KIM AU - SAYF GAZALA AU - RENE RAZZAK AU - LINGHONG GUO AU - SUNITA GHOSH AU - WILSON H. ROA AU - ERIC L.R. BÉDARD TI - Non-small Cell Lung Cancer Detection Using MicroRNA Expression Profiling of Bronchoalveolar Lavage Fluid and Sputum DP - 2015 Apr 01 TA - Anticancer Research PG - 1873--1880 VI - 35 IP - 4 4099 - http://ar.iiarjournals.org/content/35/4/1873.short 4100 - http://ar.iiarjournals.org/content/35/4/1873.full SO - Anticancer Res2015 Apr 01; 35 AB - Aim: To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC). Materials and Methods: Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls. Results: Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%. Conclusion: miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC.