PT  - JOURNAL ARTICLE
AU  - MARCZAK, AGNIESZKA
AU  - DENEL-BOBROWSKA, MARTA
AU  - ŁUKAWSKA, MAŁGORZATA
AU  - OSZCZAPOWICZ, IRENA
TI  - Formamidinodoxorubicins Are more Potent than Doxorubicin as Apoptosis Inducers in Human Breast Cancer Cells
DP  - 2015 Apr 01
TA  - Anticancer Research
PG  - 1935--1940
VI  - 35
IP  - 4
4099  - http://ar.iiarjournals.org/content/35/4/1935.short
4100  - http://ar.iiarjournals.org/content/35/4/1935.full
SO  - Anticancer Res2015 Apr 01; 35
AB  - Background/Aim: The ability of five formamidinodoxorubicins to induce apoptosis of MCF-7 breast cancer cells was tested. All these compounds were modified at C-3’ and contain a formamidine group (−N=CH–NRR), with the rest of the cyclic secondary amine (HNRR) of a gradually increasing ring size. Materials and Methods: Cytotoxicity was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. To analyze apoptosis, double staining using fluorescence probes Hoechst 33258/propidium iodide (PI) and annexin V- Fluorescein isothiocyanate/PI was carried-out. Additionally, the TdT-mediated dUTP nick-end labelling test and activity of caspase 3 were determined. Results: The four tested derivatives displayed a significant increase in antiproliferative activity in comparison to doxorubicin. All of the tested derivatives induced caspase-dependent apoptosis of MCF-7 cells. Conclusion: DOX-F MOR and DOX-F PAZ analogs are more potent apoptosis inducers than doxorubicin.