RT Journal Article
SR Electronic
T1 The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1303
OP 1310
VO 35
IS 3
A1 JAN HARTINGER
A1 PAVEL VESELÝ
A1 IRENA NETÍKOVÁ
A1 EVA MATOUŠKOVÁ
A1 LUBOŠ PETRUŽELKA
YR 2015
UL http://ar.iiarjournals.org/content/35/3/1303.abstract
AB Background: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. Materials and Methods: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. Results: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. Conclusion: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment.