TY - JOUR T1 - The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU JF - Anticancer Research JO - Anticancer Res SP - 1303 LP - 1310 VL - 35 IS - 3 AU - JAN HARTINGER AU - PAVEL VESELÝ AU - IRENA NETÍKOVÁ AU - EVA MATOUŠKOVÁ AU - LUBOŠ PETRUŽELKA Y1 - 2015/03/01 UR - http://ar.iiarjournals.org/content/35/3/1303.abstract N2 - Background: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. Materials and Methods: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. Results: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. Conclusion: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment. ER -