RT Journal Article
SR Electronic
T1 Effects of Antioxidants and Pro-oxidants on Cytotoxicity of Dihydroartemisinin to Molt-4 Human Leukemia Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1867
OP 1871
VO 35
IS 4
A1 THOMAS GERHARDT
A1 ROSS JONES
A1 JUNGSOO PARK
A1 RUI LU
A1 HO WING CHAN
A1 QINGXI FANG
A1 NARENDRA SINGH
A1 HENRY LAI
YR 2015
UL http://ar.iiarjournals.org/content/35/4/1867.abstract
AB Background: The objective of the present study was to investigate how oxidative status influences the effective ness of cytotoxicity of artemisinin towards cancer cells. It is hypothesized that antioxidants would reduce, whereas pro-oxidants would enhance, cytotoxicity. Materials and Methods: Molt-4 human leukemia cells were incubated with vitamins C, E, D3, dexamethasone, or hydrogen peroxide alone or in combination with dihydroartemisinin (DHA). Concentrations of these compounds studied were similar to those achievable by oral administration. Viable cell counts were performed before (0 h) and at, 24 and 48 h after treatment. Results: Vitamin C, vitamin D3, dexamethasone, and H2O2 caused significant Molt-4 cell death. Vitamin E caused an increase in Molt-4 cell growth. Vitamin C and vitamin D3 significantly interacted with DHA at the 48-h time point and with H2O2 at both 24-h and 48-h time points. Conclusion: Cellular oxidative status could alter the potency of artemisinin in killing cancer cells.