RT Journal Article
SR Electronic
T1 Effects on DNA Repair in Human Lymphocytes Exposed to the Food Dye Tartrazine Yellow
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1465
OP 1474
VO 35
IS 3
A1 BRUNO MOREIRA SOARES
A1 TAÍSSA MAÍRA THOMAZ ARAÚJO
A1 JORGE AMANDO BATISTA RAMOS
A1 LAINE CELESTINO PINTO
A1 BRUNA MEIRELES KHAYAT
A1 MARCELO DE OLIVEIRA BAHIA
A1 RAQUEL CARVALHO MONTENEGRO
A1 ROMMEL MARIO RODRÍGUEZ BURBANO
A1 ANDRÉ SALIM KHAYAT
YR 2015
UL http://ar.iiarjournals.org/content/35/3/1465.abstract
AB Tartrazine is a food additive that belongs to a class of artificial dyes and contains an azo group. Studies about its genotoxic, cytotoxic and mutagenic effects are controversial and, in some cases, unsatisfactory. This work evaluated the potential in vitro cytotoxicity, genotoxicity and effects on DNA repair of human lymphocytes exposed to the dye. We assessed the cytotoxicity of tartrazine by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test and the response of DNA repair through comet assay (alkaline version). We used different concentrations of the dye, ranging from 0.25-64.0 mM. The results demonstrated that tartrazine has no cytotoxic effects. However, this dye had a significant genotoxic effect at all concentrations tested. Although most of the damage was amenable to repair, some damage remained higher than positive control after 24 h of repair. These data demonstrate that tartrazine may be harmful to health and its prolonged use could trigger carcinogenesis.